yloU [2018-02-13T09:39:06.000Z]

similar to alkaline-shock protein

locus: BSU15830

pI: 7.08

mw: 13.05 kDa

protein length: 120 aa

gene length: 360 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

Gene

Coordinates: 1,656,064 → 1,656,426

The protein

Protein family

• asp23 family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|FACE7176B1A1F354CF27FB30E46A1140FAFB02F0|YqhY]

Expression and Regulation

Operons

• genes: [gene|2AD4F0AA218D4B8864E43A3E501A5B7EFC6256FE|yloU]-[gene|5DE73E8005AFFD30EB84696B2C2474899C6D1982|fakA]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B375 (yloU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1374 NBRP B. subtilis, Japan]
• GP1465 (Δ''yloU''-''cat ''), available in [SW|Jörg Stülke]'s lab [pubmed|28579978]
• GP1467 (Δ''yloU''-[gene|5DE73E8005AFFD30EB84696B2C2474899C6D1982|fakA]-''cat ''), available in [SW|Jörg Stülke]'s lab [pubmed|28579978]
• BKE15830 (Δ[gene|2AD4F0AA218D4B8864E43A3E501A5B7EFC6256FE|yloU]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACACTAGTTCCTCCTTCGA, downstream forward: _UP4_AACCCGTAGTTAGGAGGAGT
• BKK15830 (Δ[gene|2AD4F0AA218D4B8864E43A3E501A5B7EFC6256FE|yloU]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACACTAGTTCCTCCTTCGA, downstream forward: _UP4_AACCCGTAGTTAGGAGGAGT

Expression vector

• GP1476 (chromosomal ''yloU''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
• pGP1330 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab
• pGP1324 (N-terminal His-tag, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

GFP fusion

• GP1472 (spc, based on [SW|pBP43]), available in [SW|Jörg Stülke]'s lab [pubmed|28579978]

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

References

• 17114254,22383849,23420519,24178028,28579978